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KMID : 1020220170170030441
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Journal of Korean Society of Dental Hygiene
2017 Volume.17 No. 3 p.441 ~ p.448
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Effect of Cimicifuga rhizoma extract on the odontoblastic differentiation of MDPC-23 cells
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Choi Byul-Bo-Ra
Kim Ji-Young
Park Sang-Rye
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Abstract
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Objectives: The purpose of this study was to examine the cell proliferation and expression of alkaline phosphatase (ALP) during the differentiation of murine odontoblast-like cells (MDPC-23) by Cimicifuga rhizoma extract. Cimicifuga rhizoma extract was prepared using 70% ethanol. Then, the cells were treated with 25, 50, 100, 150, and 200 μg of Cimicifuga rhizoma extract.
Methods: We determined the Cimicifuga rhizoma effects of MDPC-23 using WST-1 (water soluble tetrazolium salt-1) assay, ALP activity assay and histochemical staining.
Results: 25-200 μg of Cimicifuga rhizoma extract did not inhibit the growth of MDPC-23 cells; 100±0, 100±3.29, 99±4.86, 98±3.80, 98±1.73, 99±5.05% (p<0.794). 50 μg of Cimicifuga rhizoma extract stimulated ALP activity on MDPC-23; 5.1±0.20 units/μℓ (p<0.001).
Conclusions: It was proven that Cimicifuga rhizoma promoted differentiation of MDPC- 23 cells.
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KEYWORD
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Alkaline phosphatase, Cimicifuga rhizoma, Differentiation, Odontoblast
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